UF PHA6936 FINAL EXAM PREP QUESTIONS 2024WITH GUARANTEED CORRECT ANSWERS (2025)

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• uf pha6936

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• Institution UF PHA6936
• Course UF PHA6936

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._____ , _____, _____ boundaries to the scene will prevent the alteration or destruction of physical
evidence, by restricting movement, location and activity. - ANSWER-Establishing, defining, and
controlling

.______ refers to when an instrument is tested and shown to meet previously set (frequently by the
vendor) specifications.

Performance Qualification, Installation Qualification, Operational Qualification, Design Qualification -
ANSWER-Operational Qualification

."Cutoff" is a term used in screening assays. (T/F) - ANSWER-True

.1500-200cm^-1 - ANSWER-fingerprint region, spectral "fingerprint" can be used to assist in identifying a
drug

.1st Layer of Paint - ANSWER-electrocoat primer (e-coat)

.1st step of Fingerprint Detection - ANSWER-examine all surfaces and objects

.1st step of firearm examination - ANSWER-test fires are compared to each other to determine if the
weapon produces reproducible marking each time it is fired

.1st step of hair examination - ANSWER-determine if hair, examine for cuticle scales, medulla and
pigment granules

.1st step of Paint Analysis - ANSWER-visual and macroscopical evaluation, using stereomicroscope
description, and documentation of the original condition of the sample

,.2nd layer in paint - ANSWER-primer

.2nd Step of Fingerprint Detection - ANSWER-photograph and collect all indented and visible prints,
special lighting and techniques used

.2nd step of firearm examination - ANSWER-test fire is chosen and compared with the evidence

.2nd step of hair examination - ANSWER-determine if human or animal

.3R rule - ANSWER-Radial cracks form a Right angle on the Reverse side of the force

.3rd layer of paint - ANSWER-topcoat/basecoat/colorcoat

.3rd Step of Fingerprint Detection - ANSWER-searching for latent prints

.4 microscopic characteristics that can be used to determine positive or negative microcrystal test: -
ANSWER-size, habit, color and aggregation of crystals

.4th and final layer of paint - ANSWER-clearcoat

.6 components of UV-VIS Absorbance Instrument - ANSWER-light sources, wavelength selector, cuvette,
detector, signal processor, readout device

.8 different fingerprint patterns - ANSWER-radial loop, ulnar loop, double loop, central pocket loop, plain
arch, tented arch, plain whorl, accidental

.A "cutoff" is the point below which all samples are considered to be negative. (T/F) - ANSWER-True

,.A basic substance can only behave as a base when in the presence of other bases. (T/F) - ANSWER-False

.a blood spatter pattern consisting of a large number of very fine, mist-like droplets indicates that the
blood probably came from a - ANSWER-gunshot wound

.A competitive binding assay consists of placing a fixed quantity of the antibody in a tube together with a
fixed quantity of labeled drug in addition to the drug sample to be assayed. (T/F) - ANSWER-True

.A crystalline drug has a repeating arrangement of individual molecules (crystal lattice) and a
characteristically narrow melting point range. (T/F) - ANSWER-True

.A fingerprint residue that has been developed can be removed with a scalpel and DNA extracted if
necessary (T/F) - ANSWER-True

.A flammable liquid that was used to start a fire is called - ANSWER-an accelerant

.A hapten forms the primary immune response and functions to resist disease. (T/F) - ANSWER-False

.A hapten is a monoclonal antibody produced via hybridoma technology. (T/F) - ANSWER-False

.A hapten is a protein recognized by the body's B cells as being a foreign body. (T/F) - ANSWER-False

.A hapten is a small non-immunogenic molecule attached to a larger immunogenic substance forming a
new antigen that stimulates production of antibodies specific for the small molecule. (T/F) - ANSWER-
True

, .A person's ____ is usually more distinctive than their normal handwriting - ANSWER-signature

.A signal-to-noise ratio greater than ____ is generally considered acceptable for estimating the limit of
detection.

A. 1:1,B. 3:1, C. 2:1, D. Both B and C - ANSWER-D. Both B and C

.A substrate control sample helps to detect - ANSWER-interference resulting in false negatives/positives

.A swipe bloodstain pattern is the result of - ANSWER-a bloody object in motion contacting another
clean surface

.A vaginal swab that tests positive for acid phosphatase, but shows no spermatozoa on microscopic
examination should be tested further for the presence of _____. - ANSWER-P30

.Accreditation Process - ANSWER-to do this a laboratory must demonstrate that it fulfills all the
requirements est by the appropriate regulatory bodies, this is demonstrated through a laboratory audit
that is conducted by an external, independent expert. In addition, proficiency testing for certification of
each analyte must also be done. Analysis of an ukn sample done correctly. Ex. EPA lab analzyes CCl4,
benzene or styrene via GC-MS

.Acetylating Agents for GC - ANSWER-Pentafluoropropionic anhydride (PFPA), and trifluoroacetic
anhydride (TFAA), and acetic anhydride (AA), used for amines, amides, thiols, phenolic, alcohol and
aromatic rings

More stable than silyl derivatives

.Acidic substances can only behave as an acid in the presence of other acids. (T/F) - ANSWER-False

.Acids and bases are neutral compounds never encountered in an ionized state. (T/F) - ANSWER-False